Reference Gene Optimization for Circadian Gene Expression Analysis in Human Adipose Tissue
نویسندگان
چکیده
منابع مشابه
Gene expression in human brown adipose tissue.
Brown adipose tissue (BAT) has profound effects on body weight and metabolism in rodents. Recent reports show that human adults have significant amounts of BAT. Our aim was to study the gene expression profile of human BAT. Biopsies of adipose tissue with brown-red color and subcutaneous white adipose tissue (WAT) were obtained from 24 patients undergoing surgery in the thyroid region. Intrasca...
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Housekeeping genes frequently used in gene expression studies are highly regulated in human adipose tissue. To ensure a correct interpretation of results, it is critical to select appropriate reference genes. Subcutaneous (SC) and omental (OM) adipose tissue expression was analyzed from lean and obese subjects using whole genome complementary DNA (cDNA) microarrays to identify stably expressed ...
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BACKGROUND Relative quantification is a commonly used method for assessing gene expression, however its accuracy and reliability is dependent upon the choice of an optimal endogenous control gene, and such choice cannot be made a priori. There is limited information available on suitable reference genes to be used for studies involving human epicardial adipose tissue. The objective of the curre...
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ژورنال
عنوان ژورنال: Journal of Biological Rhythms
سال: 2019
ISSN: 0748-7304,1552-4531
DOI: 10.1177/0748730419883043